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"ARTICLE";"PotvinLechowiczTardif1990";"Physiological ecologists often analyze the responses of physiological or biochemical traits to environmental factors. The data for such a response curve typically are gathered by sequential sampling of the same plant or animal; analysis should explicitly allow for this repeated-measures design. The authors address statistical treatment of response curves and illustrate the correct alternatives that are available. Four different statistical methods for analyzing response curves are considered: analysis of variance with repeated measures (ANOVAR), multivariate analysis of variance with repeated measures (MANOVAR), a nonparametric split-plot analysis (NP split-plot) and parametric comparison of models fitted to the data by nonlinear regression. Analyses of the CO2 dependence of photosynthesis in the C4 grass Echinochloa crus-galli following chilling are used as examples of these different methods. ANOVAR, potentially the most powerful analysis, makes stringent assumptions about the variance-covariance structure of the data. Within limits these assumptions can be relaxed and a corrected significance level used. When the variance-covariance structure badly violates the ANOVAR assumptions, MANOVAR or NP split-plot are viable alternatives. Use of MANOVAR frequently is limited by small sample sizes and the tendency for the number of levels of the treatment factor to exceed the sample size. The NP split-plot is based on simple assumptions and could be widely used. Comparison of curves fitted by nonlinear regression is also distribution free and provides an interesting alternative when responses are amenable to fitting. -from Authors";;;;"Potvin, C. and Lechowicz, M.J. and Tardif, S.";;;;;;;;;;;;;;"Ecology";;"analysis of variance carbon dioxide photosynthesis response curve split-plot analysis Echinochloa crus-galli";;;;"00129658 (ISSN) Cited By (since 1996): 311 Export Date: 26 April 2007 Source: Scopus Language of Original Document: English";"4";;"brugerolles";"1389-1400";;;;;;;"2007.12.05";"The statistical analysis of ecophysiological response curves obtained from experiments involving repeated measures";;;"71";"1990";;