LefevreWagnerPetitEtAl2012

Référence

Lefevre, S., Wagner, S., Petit, R.J., de Lafontaine, G. (2012) Multiplexed microsatellite markers for genetic studies of beech. Molecular Ecology Resources, 12(3):484-491. (Scopus )

Résumé

European beech (Fagus sylvatica L.) is one of the economically most important broadleaved tree species in Europe and has become a model for studying climate change effects on forests. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high-throughput genotyping. Here we present the procedure used to develop two multiplex kits (8-plexes) for European beech. We paid particular attention to quality control throughout all steps of the multiplex kits development (null allele detection, error rate measurements, linkage disequilibrium). Preliminary assays suggest that the 16 amplified loci are largely devoid of null alleles and allow rapid and cost-effective genotyping of beech with low error rates. The two kits, which differ in their levels of polymorphism, most likely due to marker origin, were also informative in seven other beech species tested. © 2011 Blackwell Publishing Ltd.

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@ARTICLE { LefevreWagnerPetitEtAl2012,
    AUTHOR = { Lefevre, S. and Wagner, S. and Petit, R.J. and de Lafontaine, G. },
    TITLE = { Multiplexed microsatellite markers for genetic studies of beech },
    JOURNAL = { Molecular Ecology Resources },
    YEAR = { 2012 },
    VOLUME = { 12 },
    NUMBER = { 3 },
    PAGES = { 484-491 },
    NOTE = { cited By 26 },
    ABSTRACT = { European beech (Fagus sylvatica L.) is one of the economically most important broadleaved tree species in Europe and has become a model for studying climate change effects on forests. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high-throughput genotyping. Here we present the procedure used to develop two multiplex kits (8-plexes) for European beech. We paid particular attention to quality control throughout all steps of the multiplex kits development (null allele detection, error rate measurements, linkage disequilibrium). Preliminary assays suggest that the 16 amplified loci are largely devoid of null alleles and allow rapid and cost-effective genotyping of beech with low error rates. The two kits, which differ in their levels of polymorphism, most likely due to marker origin, were also informative in seven other beech species tested. © 2011 Blackwell Publishing Ltd. },
    AFFILIATION = { INRA, UMR 1202 BIOGECO, F-33610 Cestas, France; Université de Bordeaux, UMR 1202 BIOGECO, F-33400 Talence, France; University of Bonn, Steinmann Institut, D-53115 Bonn, Germany },
    AUTHOR_KEYWORDS = { Fagussylvatica; Microsatellites; Multiplex PCR; Polymorphism; Repeatnumber },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1111/j.1755-0998.2011.03094.x },
    SOURCE = { Scopus },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859622752&doi=10.1111%2fj.1755-0998.2011.03094.x&partnerID=40&md5=5964019fd8a884ce2acb5ef2049be865 },
}

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