BaurechtPorthFringeli2002

Reference

Baurecht, D., Porth, I. and Fringeli, U. P. (2002) A new method of phase sensitive detection in modulation spectroscopy applied to temperature induced folding and unfolding of RNase A. Vibrational Spectroscopy, 30(1):85-92. (Scopus )

Abstract

Modulation spectroscopy can be an adequate tool to separate a weak system response from a huge background absorption, provided the process under consideration enables a periodic external stimulation. Phase sensitive detection (PSD) is then used to demodulate the periodic system response. We introduce a new method of PSD that can be used in modulated excitation (ME)-FTIR spectroscopy without the need of a lock-in amplifier or spectrometer build-in hardware. An advantage of this method is that only standard procedures included in all FTIR instruments, such as the measurement of time-resolved spectra, are required. The principal mathematical formalism of the used PSD is described. As an example we show phase-resolved spectra of the amid I′ region obtained from temperature modulated FTIR spectroscopic experiments of RNase A. The advantage of the ME compared to a relaxation process is shown by the power of separation of overlapping absorption bands. © 2002 Elsevier Science B.V. All rights reserved.

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@ARTICLE { BaurechtPorthFringeli2002,
    AUTHOR = { Baurecht, D. and Porth, I. and Fringeli, U. P. },
    TITLE = { A new method of phase sensitive detection in modulation spectroscopy applied to temperature induced folding and unfolding of RNase A },
    JOURNAL = { Vibrational Spectroscopy },
    YEAR = { 2002 },
    VOLUME = { 30 },
    NUMBER = { 1 },
    PAGES = { 85--92 },
    ABSTRACT = { Modulation spectroscopy can be an adequate tool to separate a weak system response from a huge background absorption, provided the process under consideration enables a periodic external stimulation. Phase sensitive detection (PSD) is then used to demodulate the periodic system response. We introduce a new method of PSD that can be used in modulated excitation (ME)-FTIR spectroscopy without the need of a lock-in amplifier or spectrometer build-in hardware. An advantage of this method is that only standard procedures included in all FTIR instruments, such as the measurement of time-resolved spectra, are required. The principal mathematical formalism of the used PSD is described. As an example we show phase-resolved spectra of the amid I′ region obtained from temperature modulated FTIR spectroscopic experiments of RNase A. The advantage of the ME compared to a relaxation process is shown by the power of separation of overlapping absorption bands. © 2002 Elsevier Science B.V. All rights reserved. },
    COMMENT = { Cited By :27 Export Date: 17 November 2016 },
    DATABASE = { Scopus },
    KEYWORDS = { Infrared spectroscopy, Modulated excitation, Modulation spectroscopy, Phase sensitive detection, RNase A, Light amplifiers, Modulation, Signal to noise ratio, Spectrometers, Modulation spectroscopy, Phase sensitive detection, Infrared spectroscopy },
    OWNER = { Luc },
    TIMESTAMP = { 2016.11.17 },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037130543&partnerID=40&md5=43384b325ae2b93db50d504ae5cd6188 },
}

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