WangLiPorthEtAl2016

Reference

Wang, W., Li, E., Porth, I., Chen, J.-G., Mansfield, S. D., Douglas, C. J. and Wang, S. (2016) Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis. Journal of Plant Biology, 59(1):16-23. (Scopus )

Abstract

Among the R2R3 MYB transcription factors that involve in the regulation of secondary cell wall formation in Arabidopsis, MYB46 alone is sufficient to induce the entire secondary cell wall biosynthesis program. PtrMYB021, the poplar homolog of MYB46, has been reported to regulate secondary cell wall formation when expressed in Arabidopsis. We report here that spatially and temporally restricted expression of PtrMYB021 is critical for its function in regulating secondary cell wall formation. By using quantitative RT-PCR, we found that PtrMYB021 was expressed primarily in xylem tissues. When expressed in Arabidopsis under the control of PtrCesA8, but not the 35S promoter, PtrMYB021 increased secondary cell wall thickness, which is likely caused by increased lignification as well as changes in cell wall carbohydrate composition. In consistent with this, elevated expression of lignin and cellulose biosynthetic genes were observed in the transgenic plants. When expressed in Arabidopsis protoplasts as fusion proteins to the Gal4 DNA binding domain, PtrMYB021 activated the reporter gene Gal4-GUS. In summary, our results suggest that PtrMYB021 is a transcriptional activator, and spatially and temporally restricted expression of PtrMYB021 in Arabidopsis regulates secondary cell wall formation by activating a subset of secondary cell wall biosynthesis genes. © 2016, Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg.

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@ARTICLE { WangLiPorthEtAl2016,
    AUTHOR = { Wang, W. and Li, E. and Porth, I. and Chen, J.-G. and Mansfield, S. D. and Douglas, C. J. and Wang, S. },
    TITLE = { Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis },
    JOURNAL = { Journal of Plant Biology },
    YEAR = { 2016 },
    VOLUME = { 59 },
    NUMBER = { 1 },
    PAGES = { 16--23 },
    ABSTRACT = { Among the R2R3 MYB transcription factors that involve in the regulation of secondary cell wall formation in Arabidopsis, MYB46 alone is sufficient to induce the entire secondary cell wall biosynthesis program. PtrMYB021, the poplar homolog of MYB46, has been reported to regulate secondary cell wall formation when expressed in Arabidopsis. We report here that spatially and temporally restricted expression of PtrMYB021 is critical for its function in regulating secondary cell wall formation. By using quantitative RT-PCR, we found that PtrMYB021 was expressed primarily in xylem tissues. When expressed in Arabidopsis under the control of PtrCesA8, but not the 35S promoter, PtrMYB021 increased secondary cell wall thickness, which is likely caused by increased lignification as well as changes in cell wall carbohydrate composition. In consistent with this, elevated expression of lignin and cellulose biosynthetic genes were observed in the transgenic plants. When expressed in Arabidopsis protoplasts as fusion proteins to the Gal4 DNA binding domain, PtrMYB021 activated the reporter gene Gal4-GUS. In summary, our results suggest that PtrMYB021 is a transcriptional activator, and spatially and temporally restricted expression of PtrMYB021 in Arabidopsis regulates secondary cell wall formation by activating a subset of secondary cell wall biosynthesis genes. © 2016, Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg. },
    COMMENT = { Cited By :1 Export Date: 17 November 2016 },
    DATABASE = { Scopus },
    KEYWORDS = { Arabidopsis thaliana, Populus trichocarpa, PtrMYB021, R2R3 MYB, Secondary cell wall biosynthesis, Transcription factor },
    OWNER = { Luc },
    TIMESTAMP = { 2016.11.17 },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957575143&partnerID=40&md5=d6858f5e0ddc8cb9f3c36965bf8de3fd },
}

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