HeiraliThorntonAcostaEtAl2020

Référence

Heirali, A., Thornton, C., Acosta, N., Somayaji, R., Laforest-Lapointe, I., Storey, D., Rabin, H., Waddell, B., Rossi, L., Arrieta, M.C., Surette, M., Parkins, M.D. (2020) Sputum microbiota in adults with CF associates with response to inhaled tobramycin. Thorax, 75(12):1058-1064. (Scopus )

Résumé

Background Inhaled tobramycin powder/solution (TIP/S) use has resulted in improved clinical outcomes in patients with cystic fibrosis (CF) with chronic Pseudomonas aeruginosa. However, TIP/S effect on the CF sputum microbiome has not been explored. We hypothesised that TIP/S has additional 'off-target' effects beyond merely P. aeruginosa and that baseline microbiome prior to initiation of therapy is associated with subsequent patient response. Methods We drew sputum samples from a prospectively collected biobank. Patients were included if they had one sputum sample in the 18 months before and after TIP/S. Bacterial 16S rRNA gene profiling was used to characterise the sputum microbiome. Results Forty-one patients met our inclusion criteria and 151 sputum samples were assessed. At baseline, median age was 30.4 years (IQR 24.2-35.2) and forced expiratory volume in 1 (FEV 1) second was 57% predicted (IQR 44-74). Nineteen patients were defined a priori as responders having no net decrease in FEV 1 in the year following TIP/S. No significant changes were observed in key microbiome metrics of alpha (within-sample) or beta (between-sample) diversity for samples collected before and after TIP/S. However, significant beta-diversity (Bray-Curtis) differences were noted at baseline between patients based on response status. Notably, responders were observed to have a higher abundance of Staphylococcus in pretherapy baseline samples. Conclusions Our longitudinal study demonstrates that the sputum microbiome of patients with CF is relatively stable following inhaled tobramycin over many months. Intriguingly, our findings suggest that baseline microbiome may associate with patient response to TIP/S - suggesting the sputum microbiome could be used to personalise therapy. ©

Format EndNote

Vous pouvez importer cette référence dans EndNote.

Format BibTeX-CSV

Vous pouvez importer cette référence en format BibTeX-CSV.

Format BibTeX

Vous pouvez copier l'entrée BibTeX de cette référence ci-bas, ou l'importer directement dans un logiciel tel que JabRef .

@ARTICLE { HeiraliThorntonAcostaEtAl2020,
    AUTHOR = { Heirali, A. and Thornton, C. and Acosta, N. and Somayaji, R. and Laforest-Lapointe, I. and Storey, D. and Rabin, H. and Waddell, B. and Rossi, L. and Arrieta, M.C. and Surette, M. and Parkins, M.D. },
    JOURNAL = { Thorax },
    TITLE = { Sputum microbiota in adults with CF associates with response to inhaled tobramycin },
    YEAR = { 2020 },
    NOTE = { cited By 2 },
    NUMBER = { 12 },
    PAGES = { 1058-1064 },
    VOLUME = { 75 },
    ABSTRACT = { Background Inhaled tobramycin powder/solution (TIP/S) use has resulted in improved clinical outcomes in patients with cystic fibrosis (CF) with chronic Pseudomonas aeruginosa. However, TIP/S effect on the CF sputum microbiome has not been explored. We hypothesised that TIP/S has additional 'off-target' effects beyond merely P. aeruginosa and that baseline microbiome prior to initiation of therapy is associated with subsequent patient response. Methods We drew sputum samples from a prospectively collected biobank. Patients were included if they had one sputum sample in the 18 months before and after TIP/S. Bacterial 16S rRNA gene profiling was used to characterise the sputum microbiome. Results Forty-one patients met our inclusion criteria and 151 sputum samples were assessed. At baseline, median age was 30.4 years (IQR 24.2-35.2) and forced expiratory volume in 1 (FEV 1) second was 57% predicted (IQR 44-74). Nineteen patients were defined a priori as responders having no net decrease in FEV 1 in the year following TIP/S. No significant changes were observed in key microbiome metrics of alpha (within-sample) or beta (between-sample) diversity for samples collected before and after TIP/S. However, significant beta-diversity (Bray-Curtis) differences were noted at baseline between patients based on response status. Notably, responders were observed to have a higher abundance of Staphylococcus in pretherapy baseline samples. Conclusions Our longitudinal study demonstrates that the sputum microbiome of patients with CF is relatively stable following inhaled tobramycin over many months. Intriguingly, our findings suggest that baseline microbiome may associate with patient response to TIP/S - suggesting the sputum microbiome could be used to personalise therapy. © },
    AFFILIATION = { Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada; Medicine, University of Calgary, Calgary, AB, Canada; Département de Biologie, Universite de Sherbrooke, Sherbrooke, QC, Canada; Biological Sciences, University of Calgary, Calgary, AB, Canada; Microbiology, McMaster University, Hamilton, ON, Canada; Pediatrics, Calgary, AB, Canada; Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada; Medicine, McMaster University, Hamilton, ON, Canada },
    AUTHOR_KEYWORDS = { bacterial infection; bronchiectasis; cystic fibrosis },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1136/thoraxjnl-2019-214191 },
    SOURCE = { Scopus },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096011408&doi=10.1136%2fthoraxjnl-2019-214191&partnerID=40&md5=e94adbe45956269b0c657c24fd4254f3 },
}

********************************************************** *************************** FRQNT ************************ **********************************************************

Le CEF est un
regroupement stratégique du

********************************************************** *********************** Infolettre *********************** **********************************************************

Abonnez-vous à
l'Infolettre du CEF!

********************************************************** *********************** Colloque Chaire AFD ************** **********************************************************

**********************************************************

***************** Pub - Symphonies_Boreales ****************** **********************************************************

********************************************************** ***************** Boîte à trucs *************** **********************************************************

CEF-Référence
La référence vedette !

  • Voici une liste (clairement incomplète) des packages R axés sur l'écologie! N'hésitez pas à ajouter à la liste

Voir les autres...