PartidaRodriguezNievesRamirezLaforestLapointeEtAl2021

Référence

Partida-Rodriguez, O., Nieves-Ramirez, M., Laforest-Lapointe, I., Brown, E.M., Parfrey, L., Valadez-Salazar, A., Thorson, L., Morán, P., Gonzalez, E., Rascon, E., Magaña, U., Hernandez, E., Rojas-Velázquez, L., Torres, J., Arrieta, M.C., Ximenez, C., Finlay, B.B. (2021) Exposure to Parasitic Protists and Helminths Changes the Intestinal Community Structure of Bacterial Communities in a Cohort of Mother-Child Binomials from a Semirural Setting in. mSphere, 6(4):1-19. (Scopus )

Résumé

An estimated 3.5 billion people are colonized by intestinal parasites worldwide. Intestinal parasitic eukaryotes interact not only with the host but also with the intestinal microbiota. In this work, we studied the relationship between the presence of in an asymptomatic mother-child cohort from a semirural community in Mexico. Fecal samples were collected from 46 mothers and their respective children, with ages ranging from 2 to 20 months. Mothers and infants were found to be multiparasitized by Blastocystis hominis, Entamoeba dispar, Endolimax nana, Chilomastix mesnili, Iodamoeba butshlii, Entamoeba coli, Hymenolepis nana, and Ascaris lumbricoides. Sequencing of bacterial 16S rRNA and eukaryotic 18S rRNA genes showed a significant effect of parasite exposure on bacterial beta-diversity, which explained between 5.2% and 15.0% of the variation of the bacterial community structure in the cohort. Additionally, exposure to parasites was associated with significant changes in the relative abundances of multiple bacterial taxa, characterized by an increase in Clostridiales and decreases in Actinobacteria and Bacteroidales. Parasite exposure was not associated with changes in intestinal eukaryote relative abundances. However, we found several significant positive correlations between intestinal bacteria and eukaryotes, including Oscillospira with Entamoeba coli and Prevotella stercorea with Entamoeba hartmanni, as well as the cooccurrence of the fungus Candida with Bacteroides and Actinomyces, Bifidobacterium, and Prevotella copri and the fungus Pichia with Oscillospira. The parasitic exposureassociated changes in the bacterial community structure suggest effects on microbial metabolic routes, host nutrient uptake abilities, and intestinal immunity regulation in host-parasite interactions. IMPORTANCE The impact of intestinal eukaryotes on the prokaryotic microbiome composition of asymptomatic carriers has not been extensively explored, especially in infants and mothers with multiple parasitic infections. In this work, we studied the relationship between protist and helminth parasite colonization and the intestinal microbiota structure in an asymptomatic population of mother-child binomials from a semirural community in Mexico. We found that the presence of parasitic eukaryotes microbiota in an age-dependent way. Parasitic infection was associated with an abundance of the class Clostridia and decreases of Actinobacteria and Bacteroidia. Parasitic infection was not associated with changes in the eukaryote community structure. However, we observed strong positive correlations between bacterial and other eukaryote taxa, identifying novel relationships between prokaryotes and fungi reflecting interkingdom interactions within the human intestine. © 2021. Partida-Rodriguez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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@ARTICLE { PartidaRodriguezNievesRamirezLaforestLapointeEtAl2021,
    AUTHOR = { Partida-Rodriguez, O. and Nieves-Ramirez, M. and Laforest-Lapointe, I. and Brown, E.M. and Parfrey, L. and Valadez-Salazar, A. and Thorson, L. and Morán, P. and Gonzalez, E. and Rascon, E. and Magaña, U. and Hernandez, E. and Rojas-Velázquez, L. and Torres, J. and Arrieta, M.C. and Ximenez, C. and Finlay, B.B. },
    JOURNAL = { mSphere },
    TITLE = { Exposure to Parasitic Protists and Helminths Changes the Intestinal Community Structure of Bacterial Communities in a Cohort of Mother-Child Binomials from a Semirural Setting in },
    YEAR = { 2021 },
    NOTE = { cited By 0 },
    NUMBER = { 4 },
    PAGES = { 1-19 },
    VOLUME = { 6 },
    ABSTRACT = { An estimated 3.5 billion people are colonized by intestinal parasites worldwide. Intestinal parasitic eukaryotes interact not only with the host but also with the intestinal microbiota. In this work, we studied the relationship between the presence of in an asymptomatic mother-child cohort from a semirural community in Mexico. Fecal samples were collected from 46 mothers and their respective children, with ages ranging from 2 to 20 months. Mothers and infants were found to be multiparasitized by Blastocystis hominis, Entamoeba dispar, Endolimax nana, Chilomastix mesnili, Iodamoeba butshlii, Entamoeba coli, Hymenolepis nana, and Ascaris lumbricoides. Sequencing of bacterial 16S rRNA and eukaryotic 18S rRNA genes showed a significant effect of parasite exposure on bacterial beta-diversity, which explained between 5.2% and 15.0% of the variation of the bacterial community structure in the cohort. Additionally, exposure to parasites was associated with significant changes in the relative abundances of multiple bacterial taxa, characterized by an increase in Clostridiales and decreases in Actinobacteria and Bacteroidales. Parasite exposure was not associated with changes in intestinal eukaryote relative abundances. However, we found several significant positive correlations between intestinal bacteria and eukaryotes, including Oscillospira with Entamoeba coli and Prevotella stercorea with Entamoeba hartmanni, as well as the cooccurrence of the fungus Candida with Bacteroides and Actinomyces, Bifidobacterium, and Prevotella copri and the fungus Pichia with Oscillospira. The parasitic exposureassociated changes in the bacterial community structure suggest effects on microbial metabolic routes, host nutrient uptake abilities, and intestinal immunity regulation in host-parasite interactions. IMPORTANCE The impact of intestinal eukaryotes on the prokaryotic microbiome composition of asymptomatic carriers has not been extensively explored, especially in infants and mothers with multiple parasitic infections. In this work, we studied the relationship between protist and helminth parasite colonization and the intestinal microbiota structure in an asymptomatic population of mother-child binomials from a semirural community in Mexico. We found that the presence of parasitic eukaryotes microbiota in an age-dependent way. Parasitic infection was associated with an abundance of the class Clostridia and decreases of Actinobacteria and Bacteroidia. Parasitic infection was not associated with changes in the eukaryote community structure. However, we observed strong positive correlations between bacterial and other eukaryote taxa, identifying novel relationships between prokaryotes and fungi reflecting interkingdom interactions within the human intestine. © 2021. Partida-Rodriguez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. },
    AFFILIATION = { Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAM, Mexico City, Mexico; Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada; Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada; Department of Pediatrics, University of Calgary, Calgary, Alberta, Canada; Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada; Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada; Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada; Unidad de Investigación en Enfermedades Infecciosas, UMAE Pediatria, IMSS, Mexico City, Mexico; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada },
    AUTHOR_KEYWORDS = { 16S sequencing; 18S sequencing; bacteria; children; eukaryotes; helminths; Mexico; microbiota; parasites; protists },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1128/mSphere.00083-21 },
    SOURCE = { Scopus },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115040292&doi=10.1128%2fmSphere.00083-21&partnerID=40&md5=5b56be4738f8e79b3868b114e5708043 },
}

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