IsabelBoivinLevasseurEtAl1996

Référence

Isabel, N., Boivin, R., Levasseur, C., Charest, P.M., Bousquet, J., Tremblay, F.M. (1996) Occurrence of somaclonal variation among somatic embryo-derived white spruces (Picea glauca, Pinaceae). American Journal of Botany, 83(9):1121-1130.

Résumé

Four different variegata phenotypes were identified among 2270 white spruce plants [Picea glauca (Moench.) Voss.] produced over a period of 2 yr from the time of induction of embryogenic tissues. The four variegated plants differed from each other in the extent and distribution of chlorophyll-deficient needles. Light microscopy showed that variegated leaves of a selected variant consisted of a chimeral mixture of green and white cells. Electron microscopy showed that cells in completely white needles had large nuclei with predominant euchromatin, lacked large cytoplasmic vacuoles, and harbored vacuolized plastids with aberrant morphologies. Various observations suggest that the recovered variegata phenotypes reflect some kind of genetic instability of either chloroplastic or nuclear genomes. To elucidate the genetic basis of these variegata phenotypes in white spruce, three out of the four variants were subjected to randomly amplified polymorphic DNA (RAPD) analysis. Out of more than 250 RAPD markers screened, only one correlated with white needles of variegated plants. The nucleotide sequence of this DNA fragment showed no homology with any known gene, but the amplified sequence appears most likely of nuclear origin.

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@ARTICLE { IsabelBoivinLevasseurEtAl1996,
    AUTHOR = { Isabel, N. and Boivin, R. and Levasseur, C. and Charest, P.M. and Bousquet, J. and Tremblay, F.M. },
    TITLE = { Occurrence of somaclonal variation among somatic embryo-derived white spruces (Picea glauca, Pinaceae) },
    JOURNAL = { American Journal of Botany },
    YEAR = { 1996 },
    VOLUME = { 83 },
    PAGES = { 1121-1130 },
    NUMBER = { 9 },
    NOTE = { Times Cited: 24 Article English Cited References Count: 51 Vh993 },
    ABSTRACT = { Four different variegata phenotypes were identified among 2270 white spruce plants [Picea glauca (Moench.) Voss.] produced over a period of 2 yr from the time of induction of embryogenic tissues. The four variegated plants differed from each other in the extent and distribution of chlorophyll-deficient needles. Light microscopy showed that variegated leaves of a selected variant consisted of a chimeral mixture of green and white cells. Electron microscopy showed that cells in completely white needles had large nuclei with predominant euchromatin, lacked large cytoplasmic vacuoles, and harbored vacuolized plastids with aberrant morphologies. Various observations suggest that the recovered variegata phenotypes reflect some kind of genetic instability of either chloroplastic or nuclear genomes. To elucidate the genetic basis of these variegata phenotypes in white spruce, three out of the four variants were subjected to randomly amplified polymorphic DNA (RAPD) analysis. Out of more than 250 RAPD markers screened, only one correlated with white needles of variegated plants. The nucleotide sequence of this DNA fragment showed no homology with any known gene, but the amplified sequence appears most likely of nuclear origin. },
    KEYWORDS = { conifers picea pinaceae rapd fingerprinting somatic embryogenesis structural characterization variegata phenotype mariana mill bsp polymerase chain-reaction abies l karst tissue-culture norway spruce black spruce mitochondrial genome DNA amplification p-rubens embryogenesis },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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